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Serum-free medium
CHO Cell
293 Cell
Immune Cell
Stem Cell
Cell Freezing medium
Custom service
Serum
Fetal bovine serum
Newborn bovine serum
Serum screening service
Assay kit
host cell residue assay kit
Process impurities assay kit
Microorganism rapid assay kit
Cell assay
Replicable virus
Human ELISA kit
Mouse ELISA kit
Rat ELISA kit
Service
Reagent&consumable
Molecular auxiliary reagent
Auxiliary reagent for cell culture
Consumable for molecular biology
Consumable for cell culture
Taq-Antibody(MB022-0011、0012)
Overview
Taq Antibody是在PCR熱啟動(dòng)體系中用于抗Taq DNA聚合酶的抗體,通過(guò)與Taq DNA聚合酶結(jié)合抑制酶活性。
Application
specification
10 ml,50 ml
T4 Polynucleotide Kinase(MB000-5401)
Overview
本酶能夠催化將ATP上的?磷酸基團(tuán)轉(zhuǎn)移到雙鏈或單鏈DNA的5'末端。
Application
DNA或RNA的5'末端放射性標(biāo)記,以制備探針; 寡聚核苷酸的5'末端進(jìn)行磷酸化,以方便進(jìn)行連接反應(yīng); 除去寡聚核苷酸的3'末端的磷酸基團(tuán)。
specification
250U,500U,2,500U,10,000U
T4 DNA Polymerase(MB000-1571)
Overview
在模板和引物存在的條件下,T4 DNA 聚合酶能夠催化沿5′→3′方向合成 DNA。
Application
補(bǔ)平5'突出末端或切除3'突出末端; 基因點(diǎn)突變后的第二鏈合成; 采用該酶較強(qiáng)的3′→5′外切核酸酶的活性,通過(guò)置換合成法從DNA片段的3末端標(biāo)記探針。
specification
150U,750U,3,000U
T4 DNA Ligase(MB000-5391)
Overview
本酶催化相鄰DNA鏈的5'-P末端和3'-OH末端以磷酸二酯鍵結(jié)合的反應(yīng),需要ATP作輔酶。
Application
粘性末端或平末端的連接與載體的連接; 雙鏈寡聚核苷酸接頭與雙鏈DNA的連接; 位點(diǎn)特異性突變; 雙鏈DNA,RNA或RNA-DNA復(fù)合體中缺口的修復(fù); 連接酶介導(dǎo)的RNA檢測(cè)。
specification
10,000U,20,000U,100,000U,400,000U
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