OverviewThis kit uses the Taqman probe quantitative PCR method to quickly detect residual human embryonic kidney cell 293 (HEK 293) host cell DNA in intermediates, semi-finished products and finished products of various biological products and pharmaceuticals. The kit introduces an uracil-N-glycosylase (UNG) system, which can effectively remove residual contamination of PCR products and great reduce false positives caused by contamination of amplified products. The reagent components contain internal standard control (IC), we can monitor whether the reaction process is proceeding normally and eliminate sample interference; ROX is suitable for ABI fluorescence quantitative PCR instruments or other similar equipment, and plays a role in optical path correction.
This product is suitable for biology drug solutions, finished products, semi-finished products, intermediate products or process monitoring samples produced with human embryonic kidney cell 293 stain as host. To obtain a calibration curve for quantitative sample DNA, the kit contains DNA calibrators with 5 concentration gradients (300 pg/μL to 30 fg/μL) for preparation of calibration curve.
For the extraction of residual DNA from HEK293 host cells, it is recommended to use our company’s universal residual host DNA sample pretreatment kit. Before first use, it is recommended to complete a product suitability study: confirm whether there are interferences or inhibitors in the sample or sample matrix, and select appropriate sample detection dilution conditions.
ApplicationThis kit is suitable for different sample from biopharmaceutical intermediates to final products, the detection results are accurate and reliable. It can quantitatively detect 293 DNA residues. The quantitative detection range is 30 fg/μL to 300 pg/μL.
OverviewThis kit uses the Taqman probe quantitative PCR method to quickly detect residual Escherichia coli (E.coli) host DNA in intermediates, semi-finished products and finished products of various biological products and pharmaceuticals. The kit introduces an uracil-N-glycosylase (UNG) system, which can effectively remove residual contamination of PCR products and great reduce false positives caused by contamination of amplified products. The reagent components contain internal standard control (IC), we can monitor whether the reaction process is proceeding normally and eliminate sample interference; ROX is suitable for ABI fluorescence quantitative PCR instruments or other similar equipment, and plays a role in optical path correction.
This product is suitable for biology drug solutions, finished products, semi-finished products, intermediate products or process monitoring samples produced with Escherichia coli stains (such as E.coli-K12，E.coli-DH5α，E.coli-MC1061，E.coli-HB101，E.coli-AM-7，E.coli-XL1-Blue，E.coli-YK537, etc.) as host. To obtain a calibration curve for quantitative sample DNA, the kit contains DNA calibrators with 5 concentration gradients (300 pg/μL to 30 fg/μL) for preparation of calibration curve.
For the extraction of residual DNA from E.coli host bacteria, it is recommended to use our company’s universal residual host DNA sample pretreatment kit. Before first use, it is recommended to complete a product suitability study: confirm whether there are interferences or inhibitors in the sample or sample matrix, and select appropriate sample detection dilution conditions.
ApplicationThis kit is suitable for different sample from biopharmaceutical intermediates to final products, the detection results are accurate and reliable. It can quantitatively detect E.coli DNA residues. The quantitative detection range is 300 pg/μL to 30 fg/μL.
OverviewThis kit uses the Taqman probe quantitative PCR method to quickly detect residual Chinese Hamster Ovary (CHO) host cell DNA in intermediates, semi-finished products and finished products of various biological products and pharmaceuticals. The kit introduces an uracil-N-glycosylase (UNG) system, which can effectively remove residual contamination of PCR products and great reduce false positives caused by contamination of amplified products. The reagent components contain internal standard control (IC), we can monitor whether the reaction process is proceeding normally and eliminate sample interference; ROX is suitable for ABI fluorescence quantitative PCR instruments or other similar equipment, and plays a role in optical path correction.
ApplicationThis kit is suitable for different sample from biopharmaceutical intermediates to final products, the detection results are accurate and reliable. It can quantitatively detect CHO DNA residues. The quantitative detection range is 300 pg/μL to 3 fg/μL.
